|
|
|
| Registros recuperados: 992 | |
|
|
| Bustos-Obregon,Eduardo; Vargas,Álvaro. |
| A chronic toxicity bioassay was conducted with the microcrustacean Artemia salina as the testing organism for the toxic organophosphate diazinon in order to determine if the species is an appropriate indicator of pollution in aquatic environments. Tests of animal exposure to different concentrations of the toxicant were performed for 24, 48 and 72 hours after larvae hatching. Registered mortality data was used to obtain the lethal dose 50 (LD50) of the organophosphate for each exposure time, considering the immobilization of A. salina larvae as the mortality parameter. The lethal concentration (LD50) in the same exposure times was calculated by evaluating morphological changes on the three initial stages of larval... |
| Tipo: Journal article |
Palavras-chave: Diazinon; Artemia salina; Ecotoxicology; Bioassay. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300013 |
| |
|
|
| PICCIONE,GIUSEPPE; ASSENZA,ANNA; FAZIO,FRANCESCO; GIANNETTO,CLAUDIA; CAOLA,GIOVANNI. |
| Circadian rhythmicity of physiological processes in animals has been described for some variables. In order to investigate the daily rhythmicity of blood pressure, seven foals (Equus caballus) were used for 40 days after birth. Measurements of blood pressure were done by means of an oscillometric apparatus (Argus TM-7, Schiller, Barr Switzerland) with the foals in a standing position and the cuff placed around the tail. Blood pressure was recorded twice a day, 1 hour before dawn and 1 hour before dusk for the first 10 days of life, every 2 days from the 11th to the 32nd day, on the 36th and on the 40th day. Two-way repeated measures analysis of variance (ANOVA) were used to determine statistically significant differences between the mean values recorded at... |
| Tipo: Journal article |
Palavras-chave: Blood pressure; Circadian rhythm; Chronophisiology; Foals; Equus caballus. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100007 |
| |
|
| |
|
| |
|
| |
|
|
| RAMIREZ,BEATRIZ U; RETAMAL,LEIRA; VERGARA,CECILIA. |
| The well-established trophic role of CNTF upon neurons led to performing clinical trials in patients of neurodegenerative diseases. However, trials were suspended due to side effects such as severe weight loss, hyperalgesia, coughing, muscle cramps and pain. So far it is not known how CNTF triggers the problems related to skeletal muscle cramps and pain. CNTF has also been described as a myotrophic factor for denervated skeletal muscles, but the possibility that it affects innervated muscles has also been considered. Since a myotrophic factor could be a valuable tool for treatment of several muscle diseases, we studied the effects of low doses of CNTF delivered systemically by an osmotic pump, over the electrical and mechanical properties of innervated and... |
| Tipo: Journal article |
Palavras-chave: CNTF; Contraction and relaxation time; Denervation; EMG; Fibrillation; Skeletal muscle. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300003 |
| |
|
|
| OCAMPO-GARCÉS,ADRIÁN; MENA,WILSON; HERNÁNDEZ,FELIPE; CORTÉS,NELSON; PALACIOS,ADRIÁN G. |
| Rest activity pattern was studied in wild-captured males of Octodon degus (n=9), Octodon bridgesi (n=3), and Spalacopus cyanus (n=6) (Rodentia: Octodontidae). Ten-minute resolution actograms were constructed from data obtained by an automated acquisition system. After two months of habituation to a stable light-dark schedule, recordings were performed in isolation chambers under a 12: 12 Light Dark schedule. A free-running period (constant darkness) was recorded for O. bridgesi and S. cyanus. O. degus displayed a crepuscular pattern of rest activity rhythm. Entrained O. bridgesi and S. cyanus displayed nocturnal preference, with rest anticipating light phase and without crepuscular activity bouts. Under constant darkness, active phase occurred at... |
| Tipo: Journal article |
Palavras-chave: Circadian rhythms; Chronotype; Octodon; Octodontids; Spalacopus cyanus. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200003 |
| |
|
|
| Espinoza,C; Schlechter,R; Herrera,D; Torres,E; Serrano,A; Medina,C; Arce-Johnson,P. |
| Genetically Modified Organisms (GMO) could be the answer for many relevant problems affecting crops. However, improving crops through GMO is also often associated with safety concerns, environmental risks and health issues due to the presence of foreign DNA. These limitations have prompted the development of alternative technologies. Recently, cisgenesis and intragenesis have been developed as new tools aimed to modify crops. While cisgenesis involves genetic modification using a complete copy of natural genes with their regulatory elements that belong exclusively to sexually compatible plants, intragenesis refers to the transference of new combinations of genes and regulatory sequences belonging to that particular species. So far, application of... |
| Tipo: Journal article |
Palavras-chave: Cisgenesis; Intragenesis; Grapevine promoters; VvGRIP24. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400003 |
| |
|
|
| BESSERVE,MICHEL; JERBI,KARIM; LAURENT,FRANCOIS; BAILLET,SYLVAIN; MARTINERIE,JACQUES; GARNERO,LINE. |
| Classification algorithms help predict the qualitative properties of a subject's mental state by extracting useful information from the highly multivariate non-invasive recordings of his brain activity. In particular, applying them to Magneto-encephalography (MEG) and electro-encephalography (EEG) is a challenging and promising task with prominent practical applications to e.g. Brain Computer Interface (BCI). In this paper, we first review the principles of the major classification techniques and discuss their application to MEG and EEG data classification. Next, we investigate the behavior of classification methods using real data recorded during a MEG visuomotor experiment. In particular, we study the influence of the classification algorithm, of the... |
| Tipo: Journal article |
Palavras-chave: Brain computer interface; Electroencephalography; Magnetoencephalography; Visuomotor control; Support Vector Machine. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500005 |
| |
|
|
| Montoya,Fredy; Martínez,Fernando; García-Robles,María; Balmaceda-Aguilera,Carolina; Koch,Ximena; Rodríguez,Federico; Silva-Álvarez,Carmen; Salazar,Katterine; Ulloa,Viviana; Nualart,Francisco. |
| Cartilage has poor regeneration capacity due to the scarcity of endogenous stem cells, its low metabolic activity and the avascular environment. Repair strategies vary widely, including microfracture, autologous or allogenic tissue implantation, and in vitro engineered tissues of autologous origin. However, unlike the advances that have been made over more than two decades with more complex organs, including vascular, cardiac or bone tissues, similar advances in tissue engineering for cartilage repair are lacking. Although the inherent characteristics of cartilage tissue, such as the lack of vascularity and low cellular diversity, suggest that it would be one of the more simple tissues to be engineered, its functional weight-bearing role and implant... |
| Tipo: Journal article |
Palavras-chave: Cartilage; Lesion; Microfracture; Stem cells. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400015 |
| |
|
|
| MÜLLER,ILSE; MEDINA-SELBY,ANGELICA; PALACIOS,JOSE LUIS; MARTINEZ,PATRICIO; OPAZO,PATRICIO; BRUCE,ELSA; MANCILLA,MARTA; VALENZUELA,PABLO; YUDELEVICH,ARTURO; VENEGAS,ALEJANDRO. |
| We have cloned and sequenced ten Helicobacter pylori genes from a Chilean strain (CH-CTX1) including: a cytotoxin VacA fragment, a CagA fragment (A17), a species-specific protein (TsaA), urease subunits (UreA, UreB), a flagellin subunit (FlaB), heat shock proteins (HspA and HspB), adhesin (HpaA) and a lipoprotein (Lpp20). We compared their deduced amino acid sequences with the corresponding sequences from three unrelated H. pylori strains, including fully sequenced strains 26695(UK) and J99(USA), and found that eight of them (UreA, UreB, FlaB, HspA, HspB, Lpp20, TsaA and HpaA) presented more than 97.3% identity. In contrast, VacA partial sequence showed lower identity values (93.2 - 94.9%). Moreover, we found major differences in the A17 region respect to... |
| Tipo: Journal article |
Palavras-chave: Chilean H. pylori strain; PCR-cloned genes; HpaA; VacA; CagA sequences. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100010 |
| |
|
|
| WILHELM,VIVIAN; HUARACÁN,BERNARDO; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T. |
| The genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair fragment encoding a protein of 156 aminoacids. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from other prokaryotic organisms. Both proteins were expressed in E. coli as fusion proteins with thioredoxin and purified by chromatography on Ni-column. A rabbit serum against P. salmonis total proteins reacts with the recombinant HSP10 and HSP16 proteins. Similar reactivity was determined by ELISA using serum... |
| Tipo: Journal article |
|
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300013 |
| |
|
| |
|
| |
|
|
| Ali,Shabana Kouser; George Priya Doss,C; Thirumal Kumar,D; Zhu,Hailong. |
| The current state of the art in medical genetics is to identify and classify the functional (deleterious) or non-functional (neutral) single amino acid substitutions (SAPs), also known as non-synonymous SNPs (nsSNPs). The primary goal is to elucidate the mechanisms through which functional SAPs exert their effects, and ultimately interrogating this information for association with complex phenotypes. This work focuses on coagulation factors involved in the coagulation cascade pathway which plays a vital role in the maintenance of homeostasis in the human system. We developed an integrated coagulation variation database, CoagVDb, which makes use of the biological information from various public databases such as NCBI, OMIM, UniProt, PDB and SAPs... |
| Tipo: Journal article |
Palavras-chave: Coagulation cascade pathway; Coagulation factor proteins; Single amino acid substitutions; SIFT; PolyPhen 2; I-Mutant 3; Fathmm; Align GVGD; PhD-SNP; SNPs& GO; SNAP. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100035 |
| |
|
|
| Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly. |
| BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron... |
| Tipo: Journal article |
Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020 |
| |
|
| |
|
| |
|
|
| Fragaki,Konstantina; Chaussenot,Annabelle; Benoist,Jean-François; Ait-El-Mkadem,Samira; Bannwarth,Sylvie; Rouzier,Cécile; Cochaud,Charlotte; Paquis-Flucklinger,Véronique. |
| BACKGROUND: Coenzyme Q10 (CoQ10 or ubiquinone) deficiency can be due either to mutations in genes involved in CoQ10 biosynthesis pathway, or to mutations in genes unrelated to CoQ10 biosynthesis. CoQ10 defect is the only oxidative phosphorylation disorder that can be clinically improved after oral CoQ10 supplementation. Thus, early diagnosis, first evoked by mitochondrial respiratory chain (MRC) spectrophotometric analysis, then confirmed by direct measurement of CoQ10 levels, is of critical importance to prevent irreversible damage in organs such as the kidney and the central nervous system. It is widely reported that CoQ10 deficient patients present decreased quinone-dependent activities (segments I + III or G3P + III and II + III) while MRC activities... |
| Tipo: Journal article |
Palavras-chave: Mitochondrial disease; CoQ10 deficiency; Respiratory chain; Spectrophotometry; LC-MSMS. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100004 |
| |
|
|
| DA ROSA,RENATA; RUBERT,MARCELÉIA; BERTOLLO,LUIZ A C; MARTINS-SANTOS,ISABEL C; GIULIANO-CAETANO,LUCIA. |
| Karyotypes of seventeen Hoplias malabaricus specimens, collected in the fish culture station of UNOPAR (University of Northern Paraná), were analyzed. The station is in the Claro River system in the Tibagi River basin. Two distinct and coexistent karyotype forms (cytotypes) were identified, comprising either 42 chromosomes (cytotype A) or 40 chromosomes (cytotype C), both presenting metacentric and submetacentric chromosomes. In two specimens, one male and one female, it was not possible to characterize a modal diploid number because different cell lines were observed, with a predominance of 2n=41 and 2n=42 chromosomes at a frequency of 38.24% and 41.12%, respectively. The karyotype with 2n=41 showed some putative monosomic and trisomic chromosomes, while... |
| Tipo: Journal article |
Palavras-chave: Hoplias malabaricus; Chromosomal mosaicism; Coexistence of cytotypes; RAPD; Reproductive isolation. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300003 |
| |
| Registros recuperados: 992 | |
|
|
|
